HPLC of reducing sugars with postcolumn fluorescence derivatization using methoxybenzamidine.
نویسندگان
چکیده
منابع مشابه
Specific and rapid analysis of ubiquinones using Craven's reaction and HPLC with postcolumn derivatization.
A new method for the analysis of ubiquinones in various samples was developed using an HPLC system with postcolumn derivatization. Craven's reaction, a specific color reaction for the analysis of ubiquinones, was used in the system. Because the reaction progressed in organic solvents that contained ubiquinones and ethylcyanoacetate under an alkaline condition, the selectivity for ubiquinone det...
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We describe a versatile high-performance liquid-chromatographic method for determining homocysteine and other plasma sulfhydryls. Using three different procedures for preparation of plasma, we determined total, free (non-protein-bound), and reduced forms of homocysteine, cysteine, glutathione, cysteinylglycine, and gamma-glutamylcysteine in human plasma. Sample preparation involves disulfide re...
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Metabolomic studies conducted for evaluating cancer pathogenesis and progression by monitoring the amino acids metabolic balance hold great promise for assessing current and future anticancer treatments. We performed a comprehensive quantification of 21 amino acids concentrations in cultured human colorectal adenocarcinoma cells treated with the anticancer drugs 5-fluorouracil, irinotecan, and ...
متن کاملHighly selective and simple method for determination of polythiols based on liquid chromatography with postcolumn excimer fluorescence derivatization.
An LC postcolumn derivatization method for determination of polythiols has been developed. This method involves separation using reversed-phase LC, postcolumn derivatization with N-(1-pyrenyl)maleimide, and excimer fluorescence detection. Analytes with a polythiol structure were converted into corresponding polypyrene-labeled derivatives, and the derivatives exhibited intramolecular excimer flu...
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ABSTRACT Background and Objective: Measurement of amino acids is an important tool for metabolic studies and evaluation of patients’ clinical condition. The aim of this study was to analyze the plasma amino acids using reverse phase high performance liquid chromatography techniques (RP-HPLC) with pre-column derivatization by o-phthalaldehyde (OPA)...
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ژورنال
عنوان ژورنال: BUNSEKI KAGAKU
سال: 1989
ISSN: 0525-1931
DOI: 10.2116/bunsekikagaku.38.11_568